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phospho eif4e s209  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho eif4e s209
    Phospho Eif4e S209, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 535 article reviews
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    RIPA protein extracts were prepared 4h or 18h after treatment of BJAB cells or BJAB K5F cells with ⍺Fas (10 ng/ml) and run on western blots and analyzed for, (A) caspase-8 and cleaved caspase-8, (B) Caspase-3 and cleaved caspase-3, (C) the cleaved form of PARP (89 kDa) and (D) procaspase 6. Signal intensity of each protein of interest was normalized with respect to β-actin and the value is displayed under each blot. Signal intensity of each protein with respect to β-actin and normalized to lane 1 is displayed under each blot. (E) An 18h experiment was done with ⍺Fas treatment without or with IDN-6556 (10 µM) caspase inhibitor (labeled I/⍺Fas) and then analyzed for procaspase-6. The relative levels of procaspase-6 are shown after normalizing to β-actin.

    Journal: bioRxiv

    Article Title: Caspase Cleavage of Kaposi Sarcoma-Associated Herpesvirus Proteins: A role for K5 in Preventing Caspase-Mediated Cell Death during Lytic Replication

    doi: 10.1101/2025.04.08.647834

    Figure Lengend Snippet: RIPA protein extracts were prepared 4h or 18h after treatment of BJAB cells or BJAB K5F cells with ⍺Fas (10 ng/ml) and run on western blots and analyzed for, (A) caspase-8 and cleaved caspase-8, (B) Caspase-3 and cleaved caspase-3, (C) the cleaved form of PARP (89 kDa) and (D) procaspase 6. Signal intensity of each protein of interest was normalized with respect to β-actin and the value is displayed under each blot. Signal intensity of each protein with respect to β-actin and normalized to lane 1 is displayed under each blot. (E) An 18h experiment was done with ⍺Fas treatment without or with IDN-6556 (10 µM) caspase inhibitor (labeled I/⍺Fas) and then analyzed for procaspase-6. The relative levels of procaspase-6 are shown after normalizing to β-actin.

    Article Snippet: Antibodies to caspase-8 (cat# 9496, rab), caspase-7 (cat# 9492, rab), full-length caspase-6 (cat# 9762, rab), cleaved capsase-6 (cat# 9761, rab), caspase-3 (cat# 14220, rab), PARP (cat# 9532, rab), and cleaved PARP (cat# 9741, rab), were from Cell Signaling (Boston, MA).

    Techniques: Western Blot, Labeling